Biobanking of vegetable genetic resources by in vitro conservation and cryopreservation

Today, application of in vitro culture and cryopreservation presents a remarkable strategic tool to support medium- and long-term conservation of plant genetic resources. Over the last 30 years, considerable progress has been made in the development of both methods that are currently considered as ex situ conservation strategies, complementary to traditional seed banks and in-field clonal collections. More than 45,000 accessions of vegetable crops are maintained in 22 genetic resources conservation centers (biobanks), located in 16 countries and 6 continents (Europe, Asia, Africa, Oceania, North and South America). Approximately 4/5 of these accessions are maintained in vitro but cryopreservation is also constantly growing, with almost 8300 vegetable accessions stored by this method.